The Apothecary Diaries season 3 trailer reveals Eve
The second season 3 trailer confirms Eve will perform the ending theme “AIYOU” before the anime returns on 2 October.

The Apothecary Diaries season 3 has unveiled a second trailer ahead of its October return, confirming Eve as the performer behind the new ending theme, “AIYOU”. The video comes from TOHO animation’s official channel and gives the palace mystery series another sharp musical hook before Maomao’s next case begins.
What is actually going on
The trailer confirms that the third season will begin its first cour on 2 October in Japan. It also highlights Eve’s ending song, “AIYOU”, alongside the season’s continuing palace intrigue and Maomao’s increasingly dangerous investigations.
The new season is based on the light novel series by Hyuganatsu and Touko Shino. Reina Ueda joins the cast as Bai Niangniang, while yorushika performs the opening theme “Kumo o Nukekazashimo e Watashidake”. The second cour is scheduled to follow in April 2027.
Why you should care
The series already knows how to make a reveal feel like a clue, and this trailer keeps that instinct intact. Eve’s involvement gives the ending a particularly promising identity: the song should land after episodes built around Maomao’s cool-headed deductions, when the emotional consequences have had time to catch up.
More importantly, season 3 is arriving with the show’s central appeal undisturbed. Maomao remains an unusually compelling lead because her brilliance is practical rather than showy; every observation is another tool she can use to survive a court where information is more dangerous than poison.
Our opinion
The second trailer does exactly what a returning series needs: it refreshes the mood without pretending the audience needs a full recap. The confirmed Eve ending is the detail that sticks, and the combination of a 2 October start with a second cour already planned suggests a confident continuation for one of anime’s smartest ongoing mysteries.